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Functional enrichment / Enrichment bar chart

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How to read it

Enrichment bar chart

Each bar is an enriched term; by default its length is −log10 P (longer = more significant), or choose gene count or gene ratio instead.

If your table has a category column (e.g. GO BP / CC / MF, or the source database), bars are coloured and grouped by category; tick “Top N within each category” so every category is represented.

The chart does not recompute enrichment; it shows the values in your table. Terms with P = 0 are drawn at one tenth of the smallest non-zero P.

Adjusted P values (padj / FDR) are recommended; the dashed reference line is drawn at P = 0.05 by default.

Method

Bar length = −log10(P) (or gene count, or gene ratio with “k/n” converted to k ÷ n). Example GO data: Gene Ontology Consortium (2023) Genetics 224:iyad031, GOA human annotation (CC BY 4.0).

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