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A raw P column also works; say so in the legend.
Each point is a gene. The x-axis is the log2 fold change (right = up, left = down); the y-axis is -log10 of the adjusted P value, so higher means more significant.
Dashed lines mark your thresholds: points beyond both lines count as up (red) or down (blue); all others are grey.
Thresholds drive the counts, so keep them identical to those stated in your methods. Genes without an adjusted P value (for example low-count genes removed by independent filtering) are not plotted; the page shows how many rows were skipped.
A volcano plot shows statistical significance and effect size, not biological function; interpret candidate genes together with enrichment analysis and experimental validation.
Send us your data and research question and you will receive a written plan within 1 working day: analysis steps, parameter rationale, deliverables and timeline. Quoted per project.