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Sequence utilities / Sequence extraction

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How to read it

Sequence extraction

“ID list” picks sequences from the file; “Coordinates” cuts sub-regions; the two can be combined (pick, then cut).

Coordinates are 1-based and inclusive, as in genome browsers and GFF. BED files are 0-based and half-open: add 1 to the start before use.

Regions marked (-) are returned as the reverse complement (IUPAC rules); the result ID becomes “ID:start-end(-)”.

IDs not found and out-of-range coordinates are listed in the yellow notes. Copy or download the result as FASTA; the list table records the source and coordinates of each output.

Method

Coordinates: 1-based closed intervals (GFF3 specification, Sequence Ontology); reverse complement uses the IUPAC-IUB nucleotide codes (Cornish-Bowden 1985).

Data size

Runs in your browser; up to roughly 100 MB of sequence is recommended.

Need a full analysis?

Send us your data and research question and you will receive a written plan within 1 working day: analysis steps, parameter rationale, deliverables and timeline. Quoted per project.