Sequence utilities / Translation and ORF finder
Your data stays in your browser: files are read and processed on your device, never uploaded to any server, and cleared when you close the page. Free, no sign-in.
“Translate as CDS” reads from the first base and suits known coding regions; the table checks the length (multiple of 3), start/stop codons and internal stops to reveal frame or annotation errors.
“Six-frame translation” gives the protein for three frames on each strand; * marks stop codons. The longest stop-free stretch usually points to the true coding frame.
“ORF finder” looks for open reading frames from a start to a stop codon in all six frames; coordinates are on the plus strand (1-based, stop codon included), and only the longest ORF per stop codon is reported. In the figure each row is a frame and each block an ORF.
Use table 11 for bacteria, archaea and plastids and table 2 for vertebrate mitochondria. In CDS mode, alternative start codons such as GTG/TTG are output as methionine (M).
Genetic codes follow the NCBI numbering (Elzanowski & Ostell, NCBI Genetic Codes); translations match Biopython Seq.translate.
For ORF finding, up to about 10 Mb per sequence is recommended; at most 20 sequences are drawn.
Send us your data and research question and you will receive a written plan within 1 working day: analysis steps, parameter rationale, deliverables and timeline. Quoted per project.